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PDF Deciphering the Role of V88L Substitution in NDM-
Further analysis of the translated amino acid sequences of bla NDM-5 gene revealed that these point mutations resulted in the amino acid substitution involving non-active site residues viz., Valine-88-Leucine (V88L) and Methionine-154-Leucine (M154L).
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Notably, NDM-5 variants harbored V88L and M154L mutations, which are known to increase the fitness of NDM-5 variant towards host immune system and zinc starvation.
Identification of a Novel blaNDM Variant, blaNDM-33, in an Escherichia ...
The V88L, M154L, and R270H substitutions are boxed in blue. FIGURE S2 | Localization of bla NDM-20 on Escherichia coli plasmid pNDM-20 by Southern blot hybridization using a bla NDM-20 probe (A) and by S1-PFGE (B) .
Biochemical characterization of New Delhi metallo-β-lactamase variants ...
NDM-5, -17, -20, -21 and -24 variants which contain V88L substitution (Table 1), have been reported to show improved carbapenemase activity (Hornsey et al., 2011; Liu et al., 2017; Liu et al., 2018b; Liu et al., 2018a; Liu et al., 2019).
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Liu Z, Piccirilli A, Liu D, Li W, Wang Y, Shen J. Deciphering the Role of V88L Substitution in NDM-24 Metallo-β-Lactamase. Catalysts . 2019; 9(9):744. https://doi.org/10.3390/catal9090744